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The downregulated expression of chloroplast GPX1 and GPX7 results in decreased activities of Cu/ZnSOD and MnSOD under high light intensity, coupled with excessive H 2 O 2 accumulation ( Arabidopsis GPX3 may be involved in drought stress signal transduction and contribute to H 2 O 2 scavenging in guard cells by interfering with the 2C-type serine/threonine protein phosphatases, ABI1 and ABI2, during ABA signalling reactions ( Although the roles of most antioxidant enzymes in plant growth and development have been extensively investigated, the functional mechanisms of GPXs in plants remain unclear
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H 2 O 2 was measured by monitoring the 415 nm of the titanium-peroxide complex following the method described by 2 −• levels, the reaction system contained 50 mM phosphate buffer (pH 7.8) supplemented with 17 mM sulphanilic acid, 10 mM hydroxylamine hydrochloride, and 7 mM 1-naphthylamine, with absorbance determined at 530 nm ( 1 O 2 was performed by Plant Singlet Oxygen assay kit (GENMED, UAS) to measure dimethyl-4-nitrosoaniline reduction of peak absorption under 440 nm, according to the manufacturer’s instructions
