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BHK-7—with its high viral yield—represents a promising substrate for industrial-scale inactivated FMD vaccine production ( Future studies should prioritize causal validation of identified TFs: CRISPR/Cas9-mediated knockout or overexpression of candidate TFs (e.g., Runt or zf-C2H2 family members) in parental BHK-21 cells, followed by quantitative assessment of FMDV replication, will test their necessity and sufficiency for conferring proviral or antiviral phenotypes

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