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The biotinylation reagent sulpho-NHS-LC-biotin (Pierce, Rockford, IL) was dissolved in PBS and added to cells at 0.5 mg ml −1

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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole